Mattia Lorenzo Di Francesco
- Presently I am working at the Italian Institute of Technology, where I am involved in several projects whose aim is the development of new technologies for the design and amelioration of retinal prostheses. Within the European Flagship of Graphene, I am studying the possibility to insert Graphene as a component of photoactive implantable devices, in order to increase light-transduction efficiency.
- At the Institute of Neurosciences in Montpellier (INM) I characterized the electrophysiological function of Retinal-Pigment-Epithelium cells reprogrammed from human-patient-derived iPS cells. I recorded with the Patch-Clamp technique the activity of subfamilies of expressed ion channels such as L-Type Calcium channels, Calcium-activated BK Potassium channels, and Voltage-dependent CLC-2 Chloride channels.
- At the Institute of Functional Genomics (IGF) in Montpellier I characterized the physiological properties of cardiac pacemaker-like cells differentiated from skeletal muscle-derived stem cells (skMDPM cells). With this aim, I mainly used Patch-Clamp whole-cell recordings, live Confocal-microscope line-scan measurements, and Edge-detection.
- During my PhD period, I acquired knowledge on electrophysiological and biomolecular techniques that I used for biophysical characterization of gating mechanisms in viral ion channels such as PBCV-1 Kcv, Influenza-A PB1-F2 and Influenza-A M2. With this purpose, I performed Single-ion channel recordings and Macro-current measurements on Xenopous laevis oocytes and artificial membrane bilayers.
- A membrane-targeted photoswitch potently modulates neuronal firing.
DiFrancesco ML*, Lodola F*, Colombo E*, Maragliano L, Bramini M, Paternò GM, Baldelli P, Dalla Serra M, Lunelli L, Marchioretto M, Grasselli G, Cimò S, Colella L, Fazzi D, Vurro V, Eleftheriou CG, Shmal D, Maya-Vetencourt JF, Bertarelli C*, Lanzani G*, Benfenati F*. Nature Nanotechnology, under revision. *equal contribution.
- Interfacing Graphene-Based Materials with Neural Cells.
Bramini M, Alberini G, Colombo E, Chiacchiaretta M, DiFrancesco ML, Maya-Vetencourt JF, Maragliano L, Benfenati F, Cesca F. Frontiers in Systems Neuroscience 2018. doi: 10.3389/fnsys.2018.00012.
- Engineering a Ca++-sensitive (bio) sensor from the pore-module of a potassium channel.
DiFrancesco ML, Gazzarrini S, Arrigoni C, Romani G, Thiel G, Moroni A. Sensors 2015, 15, 4913-4924. doi: 10.3390/s150304913.
- Effect of Cytosolic pH on Inward Currents Reveals Structural Characteristics of the Proton Transport Cycle in the Influenza A Protein M2 in Cell-Free Membrane Patches of Xenopus oocytes.
DiFrancesco ML, Hansen U-P, Thiel G, Moroni A, Schroeder I. PLoS One 2014 9(9): e107406. doi:10.1371/journal.pone. 0107406.
- Fast and slow gating are inherent properties of the pore module of the K+ channel Kcv.
Abenavoli A, DiFrancesco ML*, Schroeder I, Epimashko S, Gazzarrini S, Hansen UP, Thiel G, and Moroni A. Journal of General Physiology 2009 (Vol. 134 N° 3: 219-229). * Abenavoli A and DiFrancesco ML contributed equally to this work.
Interfacing Graphene-Based Materials With Neural CellsFrontiers in Systems Neuroscience, vol. 12
Engineering a Ca++-sensitive (Bio)sensor from the pore-module of a potassium channelSensors, vol. 15, (no. 3), pp. 4913-4924
Effect of cytosolic pH on inward currents reveals structural characteristics of the proton transport cycle in the influenza A protein M2 in cell-free membrane patches of Xenopus oocytesPLoS ONE, vol. 9, (no. 9)
The G-protein-gated K+ channel, iKACh, is required for regulation of pacemaker activity and recovery of resting heart rate after sympathetic stimulationJournal of General Physiology, vol. 142, (no. 2), pp. 113-126
Proapoptotic influenza A virus protein PB1-F2 forms a nonselective ion channelPLoS ONE, vol. 5, (no. 6)
Fast and slow gating are inherent properties of the pore module of the K+ channel KcvJournal of General Physiology, vol. 134, (no. 3), pp. 219-229