Giorgio Grasselli

Researcher
Researcher - Marie-Curie Fellow

Research Lines

Center

Contacts

Largo Rosanna Benzi 10 – IST-NORD / CBA
+39 010 8610 206
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Social profiles

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About

APPOINTMENTS

07/2019 - present  - Researcher - Marie Skłodowska-Curie Fellow, Istituto Italiano di Tecnologia, Genova (Italy)
07/2018 - 06/2019 - Senior Postdoc, Istituto Italiano di Tecnologia, Genova (Italy)
03/2017 - 06/2018 - Staff scientist,The University of Chicago (USA)
03/2011 - 02/2017 - Post-Doctoral Scholar, The University of Chicago (USA)
01/2010 - 01/2011 - Post-Doc, Fondazione Santa Lucia, Rome (Italy)
09/2006 - 12/2009 - PhD student, Fondazione Santa Lucia, Rome (Italy)

EDUCATION

2010 - PhD in Cellular and Molecular Biology, University of Rome Tor Vergata (Italy)
2006 - MSc in Cellular and Molecular Biology, University of Rome Tor Vergata (Italy)
2004 - BSc in Cellular and Molecular Biology, University of Rome Tor Vergata (Italy)

TEACHING

10/2019 - present  - Adjunct Professor, Physiology of the Cardiovascular and Sensorimotor Systems (BIO/09, 2 CFU, 20h), BSc for Perfusionists, Univ. of Genova (Italy)
10/2019-09/2020 - Adjunct Professor, Neurophysiology (BIO/09, 1 CFU, 12h), School of Medicine, Humanitas University, Milan (Italy)

 


E-MAIL: giorgio.grasselli [ at ] iit.it

Interests

Neuroplasticity Memory Multiple sclerosis Ataxia Cerebellum

Projects

   CURRENT FUNDING

07/2019-07/2021 - EU-H2020 Marie-Curie Individual Fellowship (MSCA-IF-2018), "FunStructure - Interdependence of functional and structural plasticity in cerebellar climbing fibers in health and disease"

    PAST FUNDING

01/2016-12/2016 - Young Investigator Award for SCA Research, National Ataxia Foundation (NAF). "Role of SK channels in cerebellar Purkinje cells in the Pathophysiology of spinocerebellar ataxia"

   TEAM MEMBERS

    MAIN METHODS

  • patch-clamp electrophysiology in acute brain slices and primary neuronal cultures
  • in vivo microinjections
  • viral vectors for gene expression/silencing
  • immunohistochemistry, fluorescent/confocal microscopy and morphometric analysis

Selected Publications

 

Research papers

  1. Grasselli G*, Boele HJ*, Titley H, Bradford N, van Beers L, Jay L, Beekhof GC, Busch SE, De Zeeuw C, Schonewille M, Hansel C (2020) - SK2 channels in cerebellar Purkinje cells contribute to excitability modulation in motor learning-specific memory traces, PLoS Biol 18(1):e3000596, PMID: 31905212 [an outreach explanation here: AIRInforma 2020]
  2. DiFrancesco ML, Lodola F, Colombo E, Maragliano L, Bramini M, Paternò GM, Baldelli P, Dalla Serra M, Lunelli L, Marchioretto M, Grasselli G, Cimò S, Colella L, Fazzi D, Eleftheriou CG, Shmal D, Maya-Vetencourt JF,Bertarelli C, Lanzani G, Benfenati F (accepted)
  3. Du X, Wei C, Pastor DPH, Rao E, Li Y, Grasselli G, Godfrey J, Palmenberg AC, Andrade J, Hansel C, Gomez CM (2019) – α1ACT is essential for survival and early cerebellar programming in a critical neonatal window, Neuron 102(4):770-785.e7, PMID: 30922876
  4. Grasselli G, He Q, Wan V, Adelman JP, Ohtsuki G, Hansel C (2016) - Activity-dependent plasticity of spike pauses in cerebellar Purkinje cells, Cell Rep 14(11):2546–2553, PMID: 26972012
  5. Piochon C*, Titley HK*, Grasselli G, Simmons DH, ElgersmaY and Hansel C (2016) - Calcium threshold shift enables frequency-independent control of plasticity by an instructive signal, Proc Natl Acad Sci U S A 113(46):13221-13226, PMID: 27799554
  6. Piochon C, Kloth AD, Grasselli G, Titley HK, Nakayama H, Hashimoto K, Wan V, Simmons DH, Eissa T, Nakatani J, Cherskov A, Miyazaki T, Watanabe M, Takumi T, Kano M, Wang SSH, Hansel C (2014) - Cerebellar Plasticity and Motor Learning Deficits in a Copy Number Variation Mouse Model of Autism, Nat Commun 5:5586, PMID: 25418414 [F1000Prime Recommendation by Sahin M]
  7. Allegra Mascaro AL*, Cesare P*, Sacconi L, Grasselli G, Mandolesi G, Maco B, Knott GW, Huag L, De Paola V, Strata P and Pavone FS (2013) - In vivo single branch axotomy induces GAP-43 dependent sprouting and synaptic remodeling in cerebellar cortex, Proc Natl Acad Sci U S A 110(26):10824-9, PMID: 23754371
  8. Grasselli G*, Rossi S*, Musella A, Gentile A, Loizzo S, Muzio L, Di Sanza C, Errico F, Musumeci G, Haji N, Fresegna D, Sepman H, De Chiara V, Furlan R, Martino G, Usiello A, Mandolesi G, Centonze D (2013) - Abnormal NMDA receptor function exacerbates experimental autoimmune encephalomyelitis, Br J Pharmacol 168(2):502-17, PMID: 22924679
  9. Mandolesi G*, Grasselli G*, Musella A, Sepman H, Musumeci G, Gentile A, Haji N, Fresegna D, Bernardi G, Centonze D (2012) - GABAergic signaling and connectivity on Purkinje cells are impaired in experimental autoimmune encephalomyelitis, Neurobiol Dis 46(2):414-24 PMID: 22349452
  10. Grasselli G§, Mandolesi G, Strata P and Cesare P (2011) – Impaired sprouting and axonal atrophy in cerebellar climbing fibres following in vivo silencing of GAP-43, PLoS One 6(6): e20791, PMID: 21695168
  11. Musumeci G*, Grasselli G*, Rossi S, De Chiara V, Musella A, Motta C, Studer V, Maccarrone M, Bernardi G, Haji N, Sepman H, Fresegna D, Mandolesi G, Centonze D (2011) - Transient receptor potential vanilloid 1 channels modulate the synaptic effects of TNF-α and of IL-1β in experimental autoimmune encephalomyelitis, Neurobiol Dis 43(3):669-77, PMID: 21672630

Review papers

  1. Grasselli G, Hansel C (2014) – Cerebellar Long-Term Potentiation: Cellular Mechanisms and Role in Learning, Int Rev Neurobiol 117C:39-51, PMID: 25172628 (book chapter)
  2. Grasselli G§, Strata P (2013) – Structural plasticity of climbing fibers and the growth-associated protein GAP-43, Front Neural Circuits 7:25, PMID: 23441024

*Equal contribution
§Corresponding author

Full list of publications (PubMed): https://www.ncbi.nlm.nih.gov/sites/myncbi/giorgio.grasselli.1/bibliography/9331719/public/?sort=date&direction=descending

Scopus AuthorID: 35573436500

ResearcherID: G-5094-2012

IIT Publications

  • 2021
  • Musto M., Bergamini M., La Terra A., Marte A., Benfenati F., Grasselli G.iit

    Activity-dependent structural plasticity of cerebellar climbing fibers and its functional consequences

    SINS 2021, Italian Society for Neuroscience
  • Musto M., Bergamini M., La Terra A., Marte A., Benfenati F., Grasselli G.iit

    Activity-dependent synaptic and structural plasticity of cerebellar climbing fibers

    FEPS European Physiology Day Virtual Meeting 2021
  • Musto M., Bergamini M., La Terra A., Marte A., Benfenati F., Grasselli G.iit

    Cerebellar climbing fibers can undergo activity-dependent structural plasticity

    71st SIF National Congress, Italian Society of Physiology
  • Musto M., Bergamini M., La Terra A., Benfenati F., Grasselli G.iit

    Modulation of climbing fibers intrinsic excitability can reshape the architecture of the olivo-cerebellar circuit

    44th Annual Meeting of the Japan Neuroscience Society
  • Bonassi G., Pelosin E., Lagravinese G., Bisio A., Grasselli G.iit, Bove M., Avanzino L.

    Somatosensory afferent inputs modulate the excitability of cerebello-cortical interaction

    Clinical Neurophysiology
  • 2020
  • DiFrancesco M.L.iit, Lodola F.iit, Colombo E.iit, Maragliano L.iit, Bramini M.iit, Paterno G.M.iit, Baldelli P., Serra M.D., Lunelli L., Marchioretto M., Grasselli G.iit, Cimo S.iit, Colella L., Fazzi D., Ortica F., Vurro V.iit, Eleftheriou C.G.iit, Shmal D.iit, Maya-Vetencourt J.F.iit, Bertarelli C.iit, Lanzani G.iit, Benfenati F.iit
    DOI

    Neuronal firing modulation by a membrane-targeted photoswitch

    Nature Nanotechnology, vol. 15, (no. 4), pp. 296-306
  • Grasselli G., Boele H.-J., Titley H.K., Bradford N., van Beers L., Jay L., Beekhof G.C., Busch S.E., De Zeeuw C.I., Schonewille M., Hansel C.
    DOI

    SK2 channels in cerebellar Purkinje cells contribute to excitability modulation in motor-learning-specific memory traces

    PLoS Biology, vol. 18, (no. 1)
  • 2019
  • Grasselli G.iit, Boele H.J., Titley H., Bradford N., van Beers L., Jay L., De Zeeuw C.I., Schonewille M., Hansel C.

    Specific learning deficit associated with selective impairments of excitability modulation dependent on SK2 channels in cerebellar Purkinje cells

    42nd Annual Meeting of the Japan Neuroscience Society
  • Grasselli G., Boele H.J., Titley H., Bradford N., van Beers L., Jay L., De Zeeuw C.I., Schonewille M., Hansel C.

    Specific motor learning memory traces are affected by SK2 channels-dependent modulation of excitability in cerebellar Purkinje cells

    IBRO World Congress of Neuroscience
  • Du X., Wei C., Hejazi Pastor D.P., Rao E.R., Li Y., Grasselli G.iit, Godfrey J., Palmenberg A.C., Andrade J., Hansel C., Gomez C.M.
    DOI

    α1ACT Is Essential for Survival and Early Cerebellar Programming in a Critical Neonatal Window

    Neuron, vol. 102, (no. 4), pp. 770-785.e7
  • 2018
  • Grasselli G., Boele H.J., Titley H., Bradford N., van Beers L., Jay L., De Zeeuw C.I., Schonewille M., Hansel C.

    Learning deficit associated with a selective and cell-type specific impairment of intrinsic plasticity in cerebellar Purkinje cells

    Annual Meeting of the Society for Neuroscience 2018
  • Grasselli G., Boele H. J., Titley H., Bradford N., van Beers L., Jay L., De Zeeuw C.I., Schonewille M., Hansel C.

    Learning deficit associated with a selective and cell-type specific impairment of intrinsic plasticity in cerebellar Purkinje cells

    69th SIF National Congress, Italian Physiological Society
  • 2016
  • Grasselli G., He Q., Wan V., Adelman J.P., Ohtsuki G., Hansel C.
    DOI

    Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells

    Cell Reports, vol. 14, (no. 11), pp. 2546-2553
  • Piochon C., Titley H.K., Simmons D.H., Grasselli G., Elgersma Y., Hansel C.
    DOI

    Calcium threshold shift enables frequency-independent control of plasticity by an instructive signal

    Proceedings of the National Academy of Sciences of the United States of America, vol. 113, (no. 46), pp. 13221-13226
  • Catanzaro G., Pucci M., Viscomi M.T., Lanuti M., Feole M., Angeletti S., Grasselli G., Mandolesi G., Bari M., Centonze D., D'Addario C., Maccarrone M.
    DOI

    Epigenetic modifications of Dexras 1 along the nNOS pathway in an animal model of multiple sclerosis

    Journal of Neuroimmunology, vol. 294, pp. 32-40
  • 2015
  • Kloth A.D., Badura A., Li A., Cherskov A., Connolly S.G., Giovannucci A., Bangash M.A., Grasselli G., Agarikano O.P., Piochon C., Tsai P.T., Geschwind D.H., Hansel C., Sahin M., Takumi T., Worley P.F., Wang S.S.-H.
    DOI

    Cerebellar associative sensory learning defects in five mouse autism models

    eLife, vol. 4, (no. JULY 2015)
  • 2014
  • Grasselli G., Hansel C.
    DOI

    Cerebellar long-term potentiation. cellular mechanisms and role in learning

    International Review of Neurobiology, vol. 117, pp. 39-51
  • Piochon C., Kloth A.D., Grasselli G., Titley H.K., Nakayama H., Hashimoto K., Wan V., Simmons D.H., Eissa T., Nakatani J., Cherskov A., Miyazaki T., Watanabe M., Takumi T., Kano M., Wang S.S.-H., Hansel C.
    DOI

    Cerebellar plasticity and motor learning deficits in a copy-number variation mouse model of autism

    Nature Communications, vol. 5
  • 2013
  • Grasselli G., Rossi S., Musella A., Gentile A., Loizzo S., Muzio L., Di Sanza C., Errico F., Musumeci G., Haji N., Fresegna D., Sepman H., De Chiara V., Furlan R., Martino G., Usiello A., Mandolesi G., Centonze D.
    DOI

    Abnormal NMDA receptor function exacerbates experimental autoimmune encephalomyelitis

    British Journal of Pharmacology, vol. 168, (no. 2), pp. 502-517
  • He Q., Titley H., Grasselli G., Piochon C., Hansel C.
    DOI

    Ethanol affects NMDA receptor signaling at climbing fiber-Purkinje cell synapses in mice and impairs cerebellar LTD

    Journal of Neurophysiology, vol. 109, (no. 5), pp. 1333-1342
  • Gentile A., Rossi S., Studer V., Motta C., De Chiara V., Musella A., Sepman H., Fresegna D., Musumeci G., Grasselli G., Haji N., Weiss S., Hayardeny L., Mandolesi G., Centonze D.
    DOI

    Glatiramer acetate protects against inflammatory synaptopathy in experimental autoimmune encephalomyelitis

    Journal of NeuroImmune Pharmacology, vol. 8, (no. 3), pp. 651-663
  • Mandolesi G., Musella A., Gentile A., Grasselli G., Haji N., Sepman H., Fresegna D., Bullitta S., De Vito F., Musumeci G., Di Sanza C., Strata P., Centonze D.
    DOI

    Interleukin-1β alters glutamate transmission at Purkinje cell synapses in a mouse model of multiple sclerosis

    Journal of Neuroscience, vol. 33, (no. 29), pp. 12105-12121
  • Allegra Mascaro A.L., Cesare P., Sacconi L., Grasselli G., Mandolesi G., Maco B., Knott G., Huang L., De Paola V., Strata P., Pavone F.S.
    DOI

    In vivo reactive neural plasticity investigation by means of correlative two photon: Electron microscopy

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8588
  • Allegra Mascaro A.L., Cesare P., Sacconi L., Grasselli G., Mandolesi G., MacO B., Knott G.W., Huang L., De Paola V., Strata P., Pavone F.S.
    DOI

    In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex

    Proceedings of the National Academy of Sciences of the United States of America, vol. 110, (no. 26), pp. 10824-10829
  • Allegra Mascaro A.L., Cesare P., Sacconi L., Grasselli G., Mandolesi G., Maco B., Knott G.W., De Paola V., Strata P., Pavone F.S.
    DOI

    In vivo two-photon imaging of climbing fibers plasticity after laser axotomy

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8804
  • Grasselli G., Strata P.
    DOI

    Structural plasticity of climbing fibers and the growth-associated protein GAP-43

    Frontiers in Neural Circuits, (no. FEBRUARY 2013)
  • 2012
  • Mandolesi G., Grasselli G., Musella A., Gentile A., Musumeci G., Sepman H., Haji N., Fresegna D., Bernardi G., Centonze D.
    DOI

    GABAergic signaling and connectivity on Purkinje cells are impaired in experimental autoimmune encephalomyelitis

    Neurobiology of Disease, vol. 46, (no. 2), pp. 414-424
  • 2011
  • Grasselli G., Mandolesi G., Strata P., Cesare P.
    DOI

    Impaired sprouting and Axonal Atrophy in cerebellar climbing fibres following in vivo silencing of the growth-associated protein GAP-43

    PLoS ONE, vol. 6, (no. 6)
  • Rossi S., Muzio L., De Chiara V., Grasselli G., Musella A., Musumeci G., Mandolesi G., De Ceglia R., Maida S., Biffi E., Pedrocchi A., Menegon A., Bernardi G., Furlan R., Martino G., Centonze D.
    DOI

    Impaired striatal GABA transmission in experimental autoimmune encephalomyelitis

    Brain, Behavior, and Immunity, vol. 25, (no. 5), pp. 947-956
  • Allegra M.A.L., Sacconi L., Pavone F.S., Cesare P., Grasselli G., Strata P.
    DOI

    Laser axotomy on cerebellar climbing fibers

    2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
  • Musumeci G., Grasselli G., Rossi S., De Chiara V., Musella A., Motta C., Studer V., Bernardi G., Haji N., Sepman H., Fresegna D., Maccarrone M., Mandolesi G., Centonze D.
    DOI

    Transient receptor potential vanilloid 1 channels modulate the synaptic effects of TNF-α and of IL-1β in experimental autoimmune encephalomyelitis

    Neurobiology of Disease, vol. 43, (no. 3), pp. 669-677
  • 2010
  • Sacconi L., Allegra L., Buffelli M., Cesare P., Dangelo E., Gandolfi D., Grasselli G., Lotti J., Mapelli J., Strata P., Pavone F.S.
    DOI

    Brain plasticity and functionality explored by nonlinear optical microscopy

    Proceedings of SPIE - The International Society for Optical Engineering, vol. 7589
  • Mandolesi G., Grasselli G., Musumeci G., Centonze D.
    DOI

    Cognitive deficits in experimental autoimmune encephalomyelitis: Neuroinflammation and synaptic degeneration

    Neurological Sciences, vol. 31, (no. SUPPL. 2)
  • Allegra Mascaro A.L., Cesare P., Sacconi L., Grasselli G., Strata P., Pavone F.S.
    DOI

    Plasticity of climbing fibers after laser axotomy

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7548
  • 2009
  • Mandolesi G., Vanni V., Cesa R., Grasselli G., Puglisi F., Cesare P., Strata P.
    DOI

    Distribution of the SNAP25 and SNAP23 synaptosomal-associated protein isoforms in rat cerebellar cortex

    Neuroscience, vol. 164, (no. 3), pp. 1084-1096

Awards

2019 - IBRO World Congress Travel Grant, International Brain Research Organization (IBRO)
2019 - Travel award, Japan Neuroscience Society (JNS)
2018 - Young Investigator Award (Premio SIF), Physiological Society of Italy (SIF)
2016 - Travel award, Italian Society for Neuroscience (SINS)
2014 - Travel award, Italian Society for Neuroscience (SINS)


Italian Scientific National Habilitations to Associate Professor (ASN II fascia), Italian Ministry of Education, University and Research (MIUR), in:

05/2019 - Molecular Biology (05/E2)
03/2018 - Physiology (05/D1), General Pathology (06/A2), Histology (05/H2)
07/2017 - Applied Biology (05/F1)

French Qualification to Associate Professor (HDR, Maître de conférences), French Ministry of Education, in :

03/2021 - Neuroscience (Section 69)