Silvio Sacchetti

Visiting scientist
Postdoc

Contacts

+39 010 5558 534

About

Set 2011 - Apr 2014 Postdoc Associate at Yale University Dept of Internarl Medicine. New Haven, CT. USA

Jan 2009 - Mar 2010 Visiting Researcher at Yale University Dept of Internarl Medicine. New Haven, CT. USA

Nov 2007 - Mar 2011 PhD in Biochimestry, Physiology and Patology of Muscle at University G. d'Annunzio, Chieti, Italy

Dec 2008 School of Physiology at University Sapienza of Rome

Jun 2008 - Aug 2008 Visiting Student at Yale University Dept of Internarl Medicine. New Haven, CT. USA

Feb 2007 Professional Qualification for Biologist

Nov 2006 - Oct 2007 Research Training at University G. d'Annunzio, Chieti, Italy

Oct 2006 M. S. in Biology at University of Peurgia, Italy

 

Cellular Methods:                          Patch Clamp techniques: whole-cell and single channel configuration, rat and mouse hippocampal slices recordings, patch clamp on inner and outer mitochondrial membrane Establishment and maintenance of cell cultures: (primary satellite cells, hippocampal neurons and peripheral lymphocytes; muscle and neuronal cell lines). Videoimaging on single cell with fluorescent probes. Immunocytochemistry and direct/ indirect  immunofluorescence. Free radicals quantitative assays of hydrogen peroxide and superoxide anion production. Bioluminescent assays of ATP  and ADP.

Cell-Free Methods:                      Cell fractionation, isolation of total and sarcoplasmic reticulum membranes of rat/mouse skeletal muscles. Mitochondria and F1FO ATPase isolation. Protein purification and Western Blot techniques.  Enzymatic assays. Binding assays with radioligands.

 

 

Selected Publications